Objective To explore the effect of Periostin on endometrial stromal cells(ESCs)proliferation and angiogenesis and its potential molecular mechanism.Methods Female patients from January 2019 to January 2021 in Hengshui People's Hospital were selected as the research objects,and their endometrial tissues were collected.The expression of Periostin mRNA and protein in endometrial tissues of clinical samples was detected by qRT-PCR,Western blot and immunohistochemistry.ESCs were isolated from the endometrial tissues,and the effect of Periostin abnormal expression on ESCs and human umbilical vein endothelial cells(HUVECs)was analyzed by CCK-8,EdU,Transwell analysis and angiogenesis tests.The expressions of PI3K/AKT/mTOR pathway related proteins and vascular endothelial growth factor A(VEGFA)were detected by Western blot.PI3K/AKT/mTOR pathway inhibitor(LY294002)was used to transfect ESCs to determine the role of PI3K/AKT/mTOR pathway in endometrium stromal cell proliferation and angiogenesis in endometriosis.Results The expression of Periostin was up-regulated in ectopic endometrial tissues.Periostin promoted the proliferation,migration and invasion of ESCs,and promoted the angiogenesis of HUVECs.In addition,Periostin activated the PI3K/AKT/mTOR pathway.After treatment with LY294002,the promoting effect of overexpressed Periostin on ESCs and HUVECs was weakened.Conclusion Periostin promotes endometriosis progression and angiogenesis by activating the PI3K/AKT/mTOR pathway,which may provide promising therapeutic targets for endometriosis.
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