Objective To investigate the effect of nuclear factor-kappa B p65(NF-κB p65)/tumor necrosis factor-α(TNF-α)on the apoptosis of fetal membrane cells in pregnant women with preterm premature rupture of membranes(PPROM).Methods 57 pregnant women who delivered in the Affiliated Loudi Hospital,University of South China from June 2021 to June 2022 were selected as the research objects.27 PPROM pregnant women were included in the experimental group,and 30 pregnant women with normal term delivery were included in the control group,and fetal membrane tissue was collected at delivery.The tissue structure of the fetal membrane was observed by HE staining.Immunohistochemical detection of fetal membrane NF-κB p65 and phosphorylated NF-κB p65(p-NF-κB p65).TUNEL was applied to detect cell apoptosis in fetal membrane tissue.The expression of NF-κB p65,p-NF-κB p65,TNF-α and cysteinyl aspartate specific proteinase-3(Caspase-3)protein expression levels were detected by Western Blotting.The mRNA expression of NF-κB p65,TNF-α and Caspase-3 in fetal membrane tissue was detected by qRT-PCR.The correlation between the expression level of p-NF-κB p65/NF-κB p65,TNF-α protein and the fetal cell apoptosis index in PPROM was analyzed.Results HE staining showed that the structure of fetal membrane tissue in control group was complete,while the structure of fetal membrane tissue was disordered,the collagen fiber layer was thin,and the cytoplasm showed largely vacuolation in the experimental group.Immunohistochemical staining showed that the NF-κB p65 was mainly expressed in the cytoplasm of fetal membrane cells,and p-NF-κB p65 was mainly expressed in the nucleus of fetal membrane cells.The expression level of NF-κB p65,TNF-α,Caspase-3 proteins and mRNA,and the cell apoptosis index in the experimental group were higher than those in the control group(P<0.05).The p-NF-κB p65/NF-κB p65 protein ratio in the experimental group were significantly higher than that in the control group(P<0.01).In experimental group,the p-NF-κB p65/NF-κB p65 protein ratio was positively correlated with the cell apoptosis index(r1= 0.897,P<0.01),the p-NF-κB p65/NF-κB p65 protein ratio was positively correlated with the expression level of TNF-α(r2 = 0.835,P<0.01),and the TNF-α protein expression level was positively correlated with the cell apoptosis index(r3 =0.934,P<0.01).Conclusion NF-κB p65 may be involved in the occurrence of PPROM by promoting the expression of TNF-α and then up-regulating Caspase-3 to cause apoptosis of fetal membrane cells.
NETL
NSTL
维普
万方数据
