The expression and clinical significance of CD47 in cervical lesion tissues
Objective To investigate the expression and clinical significance of CD47 in cervical lesion tissues at the mRNA and protein levels.Methods The expression level of CD47 mRNA in 40 cervical cancer tissues,40 HSIL tissues and 40 normal cervical tissues were detected by qRT-PCR.The expression of CD47 protein in 60 cases of cervical cancer,30 cases of HSIL and 30 normal cervical tissues was detected by IHC.The correlation between the expression level and clinical stage and differentiation degree of cervical cancer was analyzed.Kaplan-Meier curve was drawn and LogRank test was used to compare postoperative survival of patients with different expressions of CD47 protein.Cox proportional risk model was used to analyze prognostic factors of patients with cervical cancer.Results Compared with normal cervical tissues,CD47 mRNA was highly expressed in cervical cancer and HSIL(P<0.05),but there was no significant difference between cervical cancer and HSIL groups(P>0.05).Compared with normal cervical tissues,the expression level of CD47 protein in cervical cancer and HSIL tissues was increased(P<0.05),but there was no significant difference between cervical cancer and HSIL groups(P>0.05).The expression level of CD47 protein was correlated with the differentiation degree of cervical cancer,HPV infection,lymph node metastasis and vascular invasion(P<0.05).Kaplan-Meier survival analysis:progression-free survival and overall survival in high CD47 protein expression group were significantly lower than those in low CD47 protein expression group(P<0.05).Cox proportional risk model:high expression of CD47 protein was an independent risk factor for the prognosis of patients with cervical cancer(P<0.05).Conclusion CD47 is highly expressed in cervical cancer and HSIL,which is a potential therapeutic target for cervical cancer and HSIL in the future.The high expression of CD47 in cervical cancer is associated with poor prognosis and may be a prognostic indicator of cervical cancer.
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