Analysis and functional exploration of cell-derived exosome microRNAs in follicular fluid
Objective To analyze the function and possible metabolic pathways of microRNAs(miRNAs)specifically expressed in follicular fluid in normal women.Methods From September 2017 to March 2018,30 patients with infertility due to simple tubal factors were selected from the Reproductive Medicine Center of Henan Provincial People's Hospital.The first tube of clear and blood-free follicular fluid was collected on the day of oocyte retrieval,and the exosomes of follicular fluid were extracted and purified as the experimental group.Meanwhile,peripheral blood of the enrolled patients was obtained to extract and purify the exosome as control groups.And then,the extracted exosomal miRNA in the two groups were detected using miRNA microarray analysis,and the specific miRNAs in follicular fluid were screened out.Bioinformatics technology was used to analyze the functions of the specific miRNAs in follicular fluid and the possible regulatory pathways involved.Results The target genes of follicle-specific exosome miRNAs were mainly enriched in 40 signaling pathways,some of which were closely related to ovum development,such as MAPK signaling pathway,insulin signaling pathway,TGF-beta,PI3K-Akt signaling pathway,etc.PPI network interaction analysis was performed on the target genes of MAPK and TGF-β signal,and the target genes with high correlation with MAPK signal pathway were screened out as FGFR1,KRAS,BDNF,BRAF,EGFR and IGF1R.SMAD2,ACVR1,BVP4,SMAD4 and TGFBR1 were the target genes with high correlation with TGF-beta signaling pathway.Conclusions This study suggests that follicular fluid specific exosome miRNAs may play a crucial role in regulating ovum maturation,granulosa cell proliferation and apoptosis,and steroid hormone formation.
NETL
NSTL
万方数据
