Effect of miR-125b on proliferation and invasion of epithelial ovarian cancer cells via CDK14
Objective To investigate the targeting effect of miR-125b on CDK14 and its effect on proliferation and invasion of epithelial ovarian cancer(EOC)cells.Methods RT-PCR was used to detect the expression of miR-125b in EOC cell lines(OVCAR3,HO8910,SKOV3)and human ovarian epithelial cells(HOSEpiC).CCK-8 assay and colony formation assay were used to detect cell proliferation activity,and cell scratch test and Transwell chamber were used to detect cell migration and invasion ability.Double luciferase reporter gene analysis was used to verify the targeting effect of miR-125b on CDK14.Results The relative expression of miR-125b in HOSEpiC was significantly higher than that in the three EOC cell lines(P<0.05).Compared with the control group,the proliferation,migration and invasion ability of EOC cell lines were significantly decreased after treatment with miR-125b(P<0.05).After intervention with miR-125b inhibitor,the proliferation,invasion and migration of cells were significantly enhanced(P<0.05).Compared with the control group,the expression of CDK14 in miR-125b overexpression group was lower(P<0.05),the expression of CDK14 in miR-125b inhibitor group was higher(P<0.05),the cell proliferation and invasion ability of CDK14 overexpression group was stronger(P<0.05).Conclusion miR-125b can inhibit the proliferation and invasion of EOC cells by targeting CDK14.
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