Correlation analysis of p16 and Ki67 expression with persistent HPV infection after LEEP in patients with cervical intraepithelial neoplasia
Objective To analyze the correlation between p16 and Ki67 expression and persistent HPV infection after LEEP in patients with cervical intraepithelial neoplasia(CIN).Methods 169 patients with CIN who underwent LEEP in Yancheng First People's Hospital from September 2019 to September 2021 were selected and underwent HPV typing before and after the operation,and immunohistochemistry was applied to detect the expression of p16 and Ki67 proteins in the tissues of CIN,and the persistence of HPV infection was counted at 3 months after the operation,to analyze the correlation between the expression of p16 and Ki67 and the persistence of HPV infection after LEEP.Results The stronger the intensity of p16 and Ki67 expression in CIN tissues,the lower the HPV conversion rate,with the most pronounced difference between the negative expression group and the moderate-strong positive expression group(P＜0.05).The different expression levels of p16 and Ki67 in high-grade squamous intraepithelial lesions(HSIL)had a more significant impact on HPV seroconversion than in low-grade squamous intraepithelial lesions(LSIL).Whether it was HPV infection of type 16 or 18,or other types of HPV infection in cervical lesions,the stronger the expression intensity of p16 and Ki67,the lower the HPV conversion rate.The difference between the negative expression group and the moderate-strong positive expression group was the most significant(P＜0.05).In cervical lesions infected with type 16 or 18 HPV,the HPV conversion rate in the group with moderate-strong positive expression of p16 and Ki67 was significantly lower than that in the expression group with other types of HPV infection.Conclusion The expression levels of p16 and Ki67 are related to the HPV seroconversion after LEEP.Patients with high expression of p1 6 and Ki67 in CIN after LEEP should undergo long-term follow-up and receive more attention.For patients with CIN infected with HPV type 16 or 18 who undergo LEEP,regardless of the expression level of p16 and Ki67 is high or low,active long-term follow-up is necessary,while those with high expression need more attention.
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