Study on the regulation and mechanism of recombinant balsam on cell cycle,Bax,caspase-3 and Bcl-2 in cervical cancer cells
Objective To explore the regulation and mechanism of recombinant balsam on cell cycle,Bax,caspase-3 and Bcl-2 of cervical cancer cells.Methods 4 cervical cancer Hela cell lines were cultured and divided into 44 individual samples,11 of which were used as control for routine culture,and the remaining 33 were used as study group for intervention with 10,50,100 μg/mL recombinant balsamin,respectively.Apoptosis,cell cycle,caspase activity,gene expression of Bax,caspase-3,Bcl-2 and gene expression of GPR78 and CHOP were observed.Results The survival rate of Hela cells in the study group was significantly lower than that in the control group,and the difference among the three groups was statistically significant(P＜0.05).After DAPI staining,cells treated with recombinant balsamin showed apoptotic bodies(nucleus fragmentation and aggregation),while untreated or control cells did not show obvious apoptosis.The proportion of G0/G1 cycle cells in the study group was significantly higher than that in the control group,and the proportion of S and M cycle cells was significantly lower than that in the control group in a dose-dependent manner(P＜0.05).The activities of caspase-3,caspase-8 and caspase-9 and the relative expression levels of Bax,casepase-3,GPR78 and CHOP genes in the study group were significantly higher than those in the control group,while the relative expression levels of Bcl-2 genes in the study group were significantly lower than those in the control group,with dose-dependent changes(P＜0.05).Conclusion Recombinant balanin can promote apoptosis of cervical cancer cells by regulating cell cycle,caspase activity,Bax,caspase-3 and Bcl-2 gene expression,and can also regulate the expression of GPR78 and CHOP genes and induce endoplasmic reticulum stress activation.
NETL
NSTL
万方数据
