人脐带间充质干细胞对小鼠子宫内膜炎的疗效研究
Therapeutic effect of human umbilical cord mesenchymal stem cells on endometritis in mice
王彩艺 1李婧逸 2赵铭琪 3周博 3马军 2宋玉霞1
作者信息
- 1. 450014 河南 郑州,郑州大学第二附属医院生殖医学中心
- 2. 450014 河南 郑州,郑州大学第二附属医院检验科
- 3. 450014 河南 郑州,郑州大学第二附属医院医学研究中心
- 折叠
摘要
目的 探讨人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,hUC-MSCs)作用于子宫内膜炎小鼠模型的体内效应.方法 用脂多糖(lipopolysaccharide,LPS)诱导小鼠子宫内膜炎急性炎症模型,分组:对照组(PBS)、模型组(LPS)、治疗早期组(LPS+hUC-MSCs,6 h)、治疗晚期组(LPS+hUC-MSCs,12 h).肉眼观察小鼠子宫大体形态学改变,HE染色法观察子宫内膜组织学变化,ELISA法分别检测小鼠子宫炎症指标MPO及相关免疫指标TNF-α、IL-6和IL-1β的变化.结果 与对照组相比,模型组肉眼观察和组织学镜检炎症表现显著,且子宫组织中MPO、TNF-α、IL-1β和IL-6的含量显著增加.hUC-MSCs组大体和组织学表现炎症反应减轻,MPO、TNF-α、IL-1β和IL-6的含量呈不同程度下降.炎症形成后,早期灌注hUC-MSCs炎症指标下降最明显.结论 直接宫腔灌注hUC-MSCs能够缓解LPS诱导的子宫内膜炎.
Abstract
Objective To investigate the effect of human umbilical cord mesenchymal stem cells(hUC-MSCs)in repairing the mice model of endometritis in vivo.Methods Lipopolysaccharide(LPS)was used to induce acute inflammation in mice model of endometritis,grouping:control group(PBS),model group(LPS),early treatment group(LPS+hUC-MSCs,6 h),and advanced treatment group(LPS+hUC-MSCs,12 h).Morphological changes of mouse uterus were directly observed,histological changes of endometrium were observed by HE staining,ELISA method for detecting mouse uterine inflammation index MPO and related immune index TNF-α,IL-6 and IL-1β changes.Results Compared with control group,the model group showed significant inflammation through visual observation and histological examination,and the content of MPO,TNF-α,IL-1β,and IL-6 in uterine tissue significantly increased.hUC-MSCs treatment group showed reduced inflammatory reactions in morphological and histological appearance,and the value of MPO,TNF-α,IL-1β and IL-6 showed a different degree of decline.After the formation of inflammation,early perfusion of hUC-MSCs showed the most significant decrease in inflammatory indicators.Conclusion Direct intrauterine infusion of hUC-MSCs can alleviate LPS induced endometritis.
关键词
人脐带间充质干细胞/小鼠子宫内膜炎急性炎症模型/脂多糖Key words
human umbilical cord mesenchymal stem cells/acute inflammatory model of endometritis in mice/lipopolysaccharide引用本文复制引用
基金项目
河南省重点研发与推广专项(科技攻关)项目(212103210048)
出版年
2024
NETL
NSTL
万方数据