Expression and prognostic value of IL-33 and IL-13 in cervical cancer
Objective To investigate the expression of intereukin-33(IL-33)and intereukin-13(IL-13)in patients with cervical cancer and their prognostic value.Methods Cervical tissues from 40 cervical cancer patients admitted to the First Affiliated Hospital of Xinjiang Medical University from January 2020 to January 2022 were selected,while cervical tissues from 30 patients with cervical cancer screening negative for cervical leiomyomas were selected as controls,and the expression of IL-33 and IL-13 in cervical tissues of the two groups were compared using immunohistochemistry(IHC)and real-time fluorescence quantitative PCR(qRT-PCR)techniques.In addition,serum samples were collected from 99 patients with cervical cancer(cervical cancer group)and 50 normal healthy individuals(control group),and the expression of IL-33 and IL-13 in the two groups was detected using enzyme-linked immunosorbent assay(ELISA),and the relationship between IL-33 and IL-13 was evaluated by using the String database and Person correlation analysis.Subsequently,40 cervical cancer patients who submitted cervical cancer tissues were categorized into survival group(22 cases)and death group(18 cases)according to their 15-month survival results.Additionally,99 cervical cancer patients who provided serum samples were segregated into survival group(59 cases)and death group(40 cases)based on their 15-month survival outcomes.Survival analysis was conducted using Kaplan-Meier survival curve and log-Rank test method.The data of the two groups were compared and the binary Logistic regression and receiver operating characteristic curve(ROC)were plotted.Results ① The immunohistochemical results showed that the expression of IL-33 and IL-13 in cervical cancer tissues was positive,and the MOD value was significantly higher than that in normal cervical tissues(P＜0.05).qRT-PCR results showed that the relative mRNA expression of IL-33 and IL-13 was significantly higher than that of the control group(P＜0.001).② ELISA results showed that serum IL-33 and IL-13 in patients with cervical cancer were significantly higher than those in normal control group(P＜0.001).String database showed that IL-33 interacted with IL-13.Person correlation analysis showed that IL-33 was positively correlated with IL-13(P＜0.05,r=0.6382).③ Binary Logistic regression analysis showed that mass greater than or equal to 4 cm,increased PRL,IL-33 and IL-13 were independent risk factors for death in cervical cancer patients(all P＜0.05).④ Receiver operating characteristic(ROC)curve analysis showed that the area under the curve(AUC)of IL-33 and IL-13 in predicting the survival outcome of cervical cancer patients were 0.842 and 0.899,respectively.The optimal cut-off values were 31.385 pg/mL and 10.772 pg/mL.Conclusion IL-33 and IL-13 are highly expressed in both tissue and serum in patients with cervical cancer,and they are helpful to predict the prognosis of patients with cervical cancer,and provide evidence for clinical therapy.
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