摘要
目的 探究穴位埋线对多囊卵巢综合征大鼠脂代谢、卵巢功能及p53/MAPK通路的影响.方法 将大鼠随机分为空白组、模型组、穴位埋线组、二甲双胍组和3MA组,测定血清FINS、FBG水平并计算HOMA-IR,ELISA检测血清中FSH、T、LH、E2水平及血脂指标HDL-C、LDL-C、TC、TG水平,HE染色检测卵巢组织病理学变化,蛋白质印迹法检测卵巢组织中LC3Ⅱ、LC3 Ⅰ、p53、p-AMPK、AMPK蛋白表达.结果 和空白组相比,模型组大鼠体质量、FBG、FINS、HOMA-IR、血清中T、LH、FSH、TC、TG、LDL-C水平及卵巢组织中LC3 Ⅱ/LC3 Ⅰ、p-AMPK/AMPK比值均明显升高,E2、HDL-C水平及p53蛋白表达均明显降低(P＜0.05);穴位埋线组、二甲双胍组和3MA组大鼠体质量、FBG、FINS、HOMA-IR、血清中T、LH、FSH、TC、TG、LDL-C水平及卵巢组织中LC3 Ⅱ/LC3 Ⅰ、p-AMPK/AMPK比值均低于模型组,E2、HDL-C水平及p53蛋白表达均高于模型组(P＜0.05).结论 穴位埋线可改善多囊卵巢综合征大鼠胰岛素抵抗和脂代谢紊乱,可能通过调节p53/AMPK通路抑制卵巢细胞自噬恢复卵巢功能.
Abstract
Objective To investigate the effects of acupoint catgut embedding on lipid metabolism,ovarian function,and p53/MAPK pathway in rats with polycystic ovary syndrome.Methods Rats were randomly divided into blank group,model group,acupoint catgut embedding group,metformin group,and 3MA group.Serum insulin(FINS)and fasting blood glucose(FBG)levels were measured,and insulin resistance index(HOMA-IR)was calculated,ELISA was used to detect serum FSH,T,LH,E2 levels,as well as blood lipid indicators HDL-C,LDL-C,TC,TG levels.HE staining was used to detect pathological changes in ovarian tissue,and protein blotting was used to detect LC3 Ⅱ,LC3 Ⅰ,p53,p-AMPK,AMPK protein expression.Results Compared with the blank group,the model group showed a significant increase in body weight,FBG,FINS,HOMA-IR,serum T,LH,FSH,TC,TG,LDL-C levels,and LC3 Ⅱ/LC3 Ⅰ,p-AMPK/AMPK ratio in ovarian tissues,while E2,HDL-C levels,and p53 protein expression were significantly decreased(P＜0.05).Body weight,FBG,FINS,HOMA-IR,serum levels of T,LH,FSH,TC,TG,LDL-C,and LC3 Ⅱ/LC3 Ⅰ,the p-AMPK/AMPK ratio in ovarian tissue of rats in acupoint catgut embedding group,metformin group,and 3MA group were lower than the model group,while E2,HDL-C levels,and p53 protein expression were higher than the model group(P＜0.05).Conclusion Acupoint catgut implantation can improve insulin resistance and lipid metabolism disorders in rats with polycystic ovary syndrome,possibly by regulating the p53/AMPK pathway to inhibit ovarian cell autophagy and restore ovarian function.
维普
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