The effect of acupoint catgut embedding on lipid metabolism,ovarian function,and p53/AMPK pathway in a polycystic ovary syndrome model
Objective To investigate the effects of acupoint catgut embedding on lipid metabolism,ovarian function,and p53/MAPK pathway in rats with polycystic ovary syndrome.Methods Rats were randomly divided into blank group,model group,acupoint catgut embedding group,metformin group,and 3MA group.Serum insulin(FINS)and fasting blood glucose(FBG)levels were measured,and insulin resistance index(HOMA-IR)was calculated,ELISA was used to detect serum FSH,T,LH,E2 levels,as well as blood lipid indicators HDL-C,LDL-C,TC,TG levels.HE staining was used to detect pathological changes in ovarian tissue,and protein blotting was used to detect LC3 Ⅱ,LC3 Ⅰ,p53,p-AMPK,AMPK protein expression.Results Compared with the blank group,the model group showed a significant increase in body weight,FBG,FINS,HOMA-IR,serum T,LH,FSH,TC,TG,LDL-C levels,and LC3 Ⅱ/LC3 Ⅰ,p-AMPK/AMPK ratio in ovarian tissues,while E2,HDL-C levels,and p53 protein expression were significantly decreased(P＜0.05).Body weight,FBG,FINS,HOMA-IR,serum levels of T,LH,FSH,TC,TG,LDL-C,and LC3 Ⅱ/LC3 Ⅰ,the p-AMPK/AMPK ratio in ovarian tissue of rats in acupoint catgut embedding group,metformin group,and 3MA group were lower than the model group,while E2,HDL-C levels,and p53 protein expression were higher than the model group(P＜0.05).Conclusion Acupoint catgut implantation can improve insulin resistance and lipid metabolism disorders in rats with polycystic ovary syndrome,possibly by regulating the p53/AMPK pathway to inhibit ovarian cell autophagy and restore ovarian function.
万方数据
维普
