Mechanism study on the repair effect of Wujia biochemical capsule on endometrium of abortion model rats
Objective To investigate the mechanism of the repair effect of Wujia biochemical capsule on the endometrium of abortion model rats.Methods Ten female rats were randomly selected as the blank control group(blank group),and the remaining 60 female rats were housed in a 2:1 ratio with 30 male rats in a combined cage.The drug abortion rat model was prepared by gavage of mifepristone and misoprostol in pregnant rats,and the 40 rats successfully modeled were randomly divided into model group,low-dose group,medium-dose group,high-dose group and positive control group,8 rats in each group.Measured the amount of uterine bleeding,coagulation indicators,and the levels of estradiol(E2),progesterone(P),fibronectin(FN),and laminin(LN)in rat serum in each group of rats;hematoxylin eosin(HE)staining was used to detect pathological changes in rat uterine tissue;protein blotting was used to detect the expression of matrix metalloproteinase-9(MMP-9)and tissue inhibitor of metalloproteinase-1(TIMP1)proteins in uterine tissue.Results Compared with the blank group,the uterine bleeding,plasma levels of PT,TT,APTT,serum levels of P,FN and LN were significantly increased and serum levels of E2 were significantly decreased in the model group(P＜0.05);compared with the model group,the uterine bleeding,plasma levels of PT,TT,APTT,serum levels of P,FN and LN were significantly decreased in the low,medium and high dose groups and the positive control group,and the serum E2 level was significantly increased(P＜0.05).The expression of MMP-9 protein in uterine tissue of rats in the model group was higher than that in the blank group,and the expression of TIMP-1 protein was lower than that in the blank group(P＜0.05).The expression of MMP-9 protein in uterine tissue of rats in low,medium and high dose groups and positive control group was lower than that in model group,and the expression of TIMP-1 protein was higher than that in model group(P＜0.05).Conclusion Wujia biochemical capsule can treat abnormal uterine bleeding in rats after medical abortion.Its mechanism may be to improve coagulation function,regulate estrogen level,regulate the balance of MMP-9 and TIPM-1 in uterine tissue,affect FN and LN levels,and accelerate the repair of damaged endometrial matrix.
万方数据
维普
