Screening of differential genes between uterine leiomyosarcoma and leiomyoma in GEO database
Objective This study aims to uncover the pathogenesis of uterine leiomyosarcoma by identifying key genes via the GEO database and clinical samples,offering new molecular markers for diagnosis and treatment. Methods The study extracted differential expression genes (DEGs) from GEO datasets GSE207350 and GSE205596,followed by GO,KEGG,DO,and Reactome analyses for enrichment in key biological functions and pathways. A protein-protein interaction ( PPI) network was constructed using STRING and Cytoscape to identify hub genes,which were validated with 128 clinical pathological specimens. Results The two datasets analysis identified 10397 DEGs,with 5875 upregulated and 4522 downregulated genes. Biological functional enrichment analysis reveals that these DEGs were mainly related to cell proliferation,adhesion,and cycle regulation. Twelve central hub genes,including ACTG2,IGF1,and CDH11,were highlighted. qPCR experiments showed significantly reduced CDH11 and SFRP4 expression and increased ACTG2 expression in uterine leiomyosarcoma tissues. However,only SFRP4 showed a significant reduction at the protein level. Conclusion This study elucidates the critical role of SFRP4 in the progression of uterine leiomyosarcoma,underscoring its potential utility as a biomarker. These findings present promising molecular targets for the advancement of diagnostic and therapeutic strategies.
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