Interaction between VDRrs4073729 gene polymorphism and perimenopausal syndrome and its effect on patients complicated with osteoporosis
Objective To explore the interaction between VDRrs4073729 gene polymorphism and perimenopausal syndrome and its effect on patients complicated with osteoporosis. Methods A total of 199 perimenopausal women and 180 healthy volunteers ( control group) who visited North China Medical and Health Group Shijiazhuang Huayao Hospital from October 2019 to January 2023 were enrolled,and perimenopausal women were divided into osteoporosis group ( n=99 ) and non-osteoporosis group ( n=100 ) according to bone density testing. The general data of the subjects in each group were collected,and the bone mineral density of the lumbar vertebrae was detected by X-ray absorptiometry. Enzyme-linked immunoassay ( ELISA) was used to determine bone alkaline phosphatase,osteocalcin and other bone metabolism indexes. SNPs at rs4073729 in VDR gene were detected by SNP genotyping PCR reagent ( probe method ),genotype and allele frequencies were calculated,and the interaction between rs4073729 SNPs and perimenopausal syndrome in VDR gene was detected by Logistic regression model. Results The age of the osteoporosis group was higher than that of the control group ( P<0.05 ),and the T value of lumbar bone mineral density,bone alkaline phosphatase and osteocalcin in non-osteoporosis group and osteoporosis group were higher than those of the control group ( P<0.05 ) . The genotype distribution of rs4073729 in non-osteoporosis group,osteoporosis group and control group conformed to Hardy-Weinberg's law ( x2=1.667,P=0.373;x2=0.133,P=0.936;x2=0.864,P=0.469). The frequency of GA/AG genotype distribution was the highest in the non-osteoporosis group and osteoporosis group,and the frequency of GG genotype distribution was the highest in the control group. Perimenopausal patients with the rs4073729-A allele of VDR gene had a higher risk of osteoporosis than healthy individuals of control group,and the incidence of disease in individuals with rs11037909-A allele polymorphism ( GA+AA) was higher than that of GG genotype,with adjusted age OR=1.728,95％ CI:1.125-2.331 (P<0.05). Logistic regression analysis showed that individuals with GA/AA genotype had a higher risk of osteoporosis than individuals with GG genotype (OR=4.105,95％CI:1.524-11.335),and individuals with perimenopausal syndrome with GG genotype had a higher risk of osteoporosis than individuals with the same genotype without perimenopausal syndrome (OR=3.626,95％CI:1.196-10.432).Conclusion The VDR gene rs4073729-A allele is a risk factor for osteoporosis in perimenopausal women,and the interaction between VDR gene rs4073729 and perimenopausal syndrome increases the susceptibility to osteoporosis,and the VDR gene rs4073729-A allele and perimenopausal syndrome are both high-risk factors for osteoporosis,and the risk of osteoporosis after the interaction between the two is higher than that of those without risk factors.
vitamin D receptorgene polymorphismperimenopausal syndromeosteoporosisinteractions
万方数据
