Exploration of the approaches to establishing excessive fu-viscera caused by phlegm-heat syndrome rat models
Objective To establish excessive fu-viscera caused by phlegm-heat syndrome rat models with different high-fat dietary patterns and explore effects of different modeling approaches on the quality of the models.Methods A total of 36 male SD rats were randomly divided into group A,group B,or group C,with 12 rats in each group.Group A was given normal maintenance feed,group B was given high-fat feed,group C was given normal maintenance feed+high-fat emulsion by gavage,and the modeling cycle lasted for 28 days.From the 26th day of modeling,the rats in groups B and C were given autologous fecal suspension by gavage for 3 consecutive days.The general indexes,indexes for tongue manifestation,fecal indexes,and blood lipids levels were compared between the three groups.Results Compared with group A,the average daily feed intake and water intake of groups B and C were lower(all P＜0.05),and there was no statistically significant difference in the average daily feed intake or water intake between groups B and C(all P＞0.05).There was no statistically significant difference in the body weight among the three groups in the 1st or 2nd week of modeling(all P＞0.05);the body weights of groups A and B were higher than that of group C in the 3rd week of modeling(all P＜0.05);the body weights of group B,A,and C decreased successively in the 4th week of modeling(all P＜0.05).In the 1st week of modeling,the body temperature of group C was lower than those of groups A and B(all P＜0.05);the body temperature of group B was higher than those of groups A and C in the 2nd week of modeling;the body temperatures of groups B and C were higher than that of group A in the 3rd week of modeling;the body temperature of group B was higher than those of groups A and C in the 4th week of modeling(all P＜0.05).After the last gavage,the R,G,and B values for tongue manifestation in groups B and C were lower than those in group A,and the sublingual venation scores of groups B and C were higher than that of group A(all P＜0.05).Compared with group A,the fecal pellets numbers,fecal wet weights,and Bristol Stool Form Scale(BSFS)scores of groups B and C were less/lower(all P＜0.05),but there was no statistically significant difference in the fecal pellets number,fecal wet weight,or BSFS score between groups B and C(all P＞0.05).After the fecal gavage,compared with group A,the serum levels of total cholesterol(TC),triglyceride(TG),and low-density lipoprotein cholesterol(LDL-C)in group B were higher(all P＜0.05),and the serum levels of TC and LDL-C in group C were higher(all P＜0.05);there was no statistically significant difference in serum level of LDL-C,TC,TG,or HDL-L between groups B and C(all P＞0.05).Conclusion Compared with the normal maintenance feed+high-fat emulsion by gavage+autologous fecal suspension by gavage,the method of high-fat feed+autologous fecal suspension by gavage can replicate an excessive fu-viscera caused by phlegm-heat syndrome rat model with more obvious signs,stronger stability,and better homogeneity.
Excessive fu-viscera caused by phlegm-heat syndromeAnimal modelHigh-fat dietsFecal suspensionRat
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维普
