Ultrasound molecular radiomics for evaluation of prostate specific membrane antigen expression in prostate cancer:Experimental study
Objective To observe the value of ultrasound molecular radiomics for evaluation of prostate specific membrane antigen(PSMA)expression in prostate cancer(PCa).Methods 22RV1 cells(PSMA positive expression,n=9)and PC-3 cells(PSMA negative expression,n=10)were inoculated subcutaneously in nude mice to construct human prostate cancer cell transplantation tumor models with different PSMA expression level.PSMA targeted ultrasound nanobubbles(PSMA-NB)were prepared,and the characterizations of PSMA-NB were detected,while in vivo and in vitro ultrasound molecular imaging were acquired.The nude mice were randomly divided into training set or test set at a ratio of 7∶3.The texture features were extracted in training set.Ultrasound molecular radiomics model was constructed after screening features using intra-class correlation coefficient,one-way analysis,least absolute shrinkage and selection operator,and its efficency for diagnosing PSMA positive PCa was evaluated.Results The average particle size of PSMA-NB was(392.2± 31.56)nm,and the Zeta potential was(-29.3±0.95)mV.The targeted nanobubbles showed good stability on in vitro ultrasound imaging.A total of 1 561 texture features were extracted in training set,and 3 features,i.e.exponential_glszm Zone Variance,wavelet_HHH_gldm_SmallDependenceLowGrayLevelEmphasis and logarithm_gldm_Dependence Variance were screened out.The area under the curve of this radiomics model was 0.950 and 0.875 in training set and test set,respectively.Conclusion Ultrasound molecular radiomics model based on targeted PSMA could be used to evaluate PSMA expression in nude mice PCa.
prostatic neoplasmsultrasonographyanimal experimentationprostate specific membrane antigen
万方数据
维普
