Analysis of diffusion tensor imaging reconstruction results of masseter muscles with different ROI
Objective To observe the reproducibility and difference of reconstruction results of diffusion tensor imaging(DTI)of masseter muscles with different methods to draw ROI,as well as the difference in DTI reconstruction results of masseter muscles under different chewing side performances.Methods Twenty-five healthy adults were prospectively enrolled,and DTI of masseter muscles were reconstructed based on single-layer ROI(ROI-1),three-layer ROI(ROI-3)or whole muscle volume,i.e.volume of interest(VOI),respectively.Intra-observer and inter-observer reproducibility,intergroup differences and intergroup correlations,as well as correlations with fat fraction(FF)of DTI reconstruction results with different ROI were evaluated.The difference of DTI reconstruction results of masseter muscles with different chewing side performances were analyzed.Results DTI reconstruction results of masseter muscles based on ROI-1,ROI-3 or VOI were all highly reproducible(all ICC>0.90).The fiber track number(FTN),mean fiber track length(MFTL),fiber track volume(FTV),mean diffusivity(MD),axial diffusivity(AD)and radial diffusivity(RD)significant different with different ROI(adjusted P<0.05),whereas positive correlations of DTI results were found with 3 different ROI(r=0.739-0.994,all P<0.05).FTN,FTV,MD,and RD with different ROI were all negatively correlated with FF(r=-0.628 0.284,all P<0.05).MFTL of the non-habitual chewing masseter muscles with various ROI were shorter than that of balanced chewing performance masseter muscles(all P<0.05).Conclusion DTI reconstruction results of masseter muscles with different ROI were significantly different but also correlated and with high reproducibility.All 3 ROI could be used to show changes of the altered fiber track length of non-habitual chewing masseter muscles.
masseter musclediffusion tensor imagingreproducibility of resultsprospective studies