大鼠血浆中奥昔布宁及其N-去乙基代谢物的UPLC-MS/MS法测定
Determination of Oxybutynin and N-Desethyloxybutynin in Rat Plasma by UPLC-MS/MS
朱壮志 1武余波 1罗华菲 1王浩 1高凡2
作者信息
- 1. 中国医药工业研究总院药物制剂国家工程研究中心,上海201203
- 2. 上海市药剂学校,上海200065
- 折叠
摘要
建立了超高效液相色谱-串联质谱法同时测定大鼠血浆中的奥昔布宁及其主要代谢物N-去乙基奥昔布宁.用液-液萃取法处理血样,以盐酸奥昔布宁-D11为内标,采用ESI源正离子模式、多反应监测进行测定.检测离子对分别为m/z 358.2→124.1(奥昔布宁)、m/z 330.2→96.1 (N-去乙基奥昔布宁)和m/z 369.3→142.1(奥昔布宁-D11).奥昔布宁及N-去乙基奥昔布宁在0.5～100 ng/ml和0.2～40 ng/ml浓度范围内线性关系良好,日内、日间RSD均小于8％.
Abstract
An UPLC-MS/MS method was established for the simultaneous determination of oxybutynin and its main metabolite iV-desethyloxybutynin in rat plasma with oxybutynin-D11 hydrochloride as the internal standard. The plasma samples were treated by liquid-liquid extraction. A tandem mass spectrometric detection was conducted under positive ionization mode with an electrospray ionization (ESI) interface. A multiple reaction monitoring (MRM) mode was chosen for the detection ofw/z 358.2→124.1 (oxybutynin), m/z 330.2→96.1 (N-desethyloxybutynin) and m/z 369.3→142.1 (oxybutynin-D11). The calibration curves were linear in the concentration range of 0.5 - 100 ng/ml for1 oxybutynin and 0.2 - 40 ng/ml for N-desethyloxybutynin, respectively. The intra- and inter-day RSDs were less than 8 % .
关键词
奥昔布宁/N-去乙基奥昔布宁/超高效液相色谱-串联质谱/测定Key words
oxybutynin/N-desethyloxybutynin/UPLC-MS/MS/determination引用本文复制引用
出版年
2012
NETL
NSTL
维普
万方数据