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耳聋丸中6种成分的HPLC-波长切换法测定

Determination of Six Compounds in Erlong Pills by HPLC with Wavelength Switching

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建立了高效液相色谱法测定耳聋丸中6种成分的方法.采用Dikma Platisil ODS色谱柱,以乙腈:0.1％磷酸为流动相,梯度洗脱,切换波长检测,检测波长分别为240 nm(京尼平龙胆双糖苷、栀子苷、木通苯乙醇苷B)、274 nm(龙胆苦苷、黄芩苷、甘草酸铵).上述6种成分均达到基线分离,在相应范围内线性关系良好,平均回收率为97.5％～104.6％,RSD为1.15％～1.83％.
An HPLC method was established for the determination of six main components in Erlong pills.A Dikma Platisil ODS column was used,with the mobile phase of acetonitrile:0.1％ phosphoric acid by gradient elution.The detection wavelength was switched from 240 nm for genipin 1-gentiobioside,geniposide and calceolarioside B to 274 nm for gentiopicrin,baicalin and ammonium glycyrrhizinate.The results showed that all the six components were well separated,and the linear relationships were good in their corresponding ranges.Their average recoveries were 97.5 ％-104.6％,with RSDs of 1.15 ％-1.83 ％.

Erlong pillHPLCwavelength switchingcontent determination

郝乘仪、昌盛

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吉林医药学院药学院药物分析教研室,吉林吉林132013

耳聋丸 高效液相色谱 波长切换 含量测定

吉林省教育厅课题

2015404

2017

10.16522/j.cnki.cjph.2017.01.018

中国医药工业杂志
上海医药工业研究院,中国化学制药工业协会

中国医药工业杂志

CSTPCDCSCD北大核心
影响因子：0.487
ISSN：1001-8255
年,卷(期)：2017.48(1)
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