Monosaccharide Fingerprint of Epimedium Polysaccharides Based on PMP Pre-column Derivatization and HPLC Method
The monosaccharide HPLC fingerprint method of Epimedium polysaccharides using acid hydrolysis by pre-column derivatization was established.The Epimedium polysaccharides were hydrolyzed into monosaccharides by 2.0 mol/L hydrochloric acid and derivatized by 1-phenyl-3-methyl-5-pyrazolone(PMP).Then,the monosaccharide components were detected by HPLC.The YMC-Pack ODS-A column(4.6 mm×250 mm,5 μm)was used with a mixture of phosphate buffer and acetonitrile as the mobile phase,in a flow rate of 0.8 mL/min and at a detection wavelength of 254 nm.The common fingerprints of forty batches of Epimedii Folium were established with ten common peaks by Similarity Evaluation System for Chromatographic Fingerprint of TCM(Version 2012).Eight peaks in which were identified as D-mannose,D-glucuronic acid,rhamnose,galacturonic acid,D-glucose,galactose,xylose and arabinose by reference substances.Similarity analysis and orthogonal partial least squares analysis were applied to investigate the differences between Epimedium and seven batches of E.wushanense T.S.Ying.The similarities were less than 0.9,and four monosaccharides showing significant differences were distinguished.The established method is simple and repeatable,which can provide references for the quality evaluation of Epimedium.
万方数据
维普
