A liquid chromatography coupled to tandem mass spectrometry(LC-MS/MS)method was established for determination of aripiprazole in Beagle dog plasma with diphenhydramine as the internal standard.The detection was conducted with an electrospray ionization(ESI)interface via multiple reaction monitoring(MRM)under positive ionization mode.It was linear for aripiprazole in the concentration range of 0.1-100 ng/mL.The method validation tests were carried out.The recoveries were 97.8％-100.3％,and intra-and inter-day RSDs were respectively less than 3.80％and 6.97％.No significant matrix effects were observed and the analytes were proved to be stable.This validated method was applied to the pharmacokinetics study of the self-made aripiprazole orodispersible films and the commercially available aripiprazole orally disintegrating tablets in Beagle Dogs.The results exhibited the similar pharmacokinetic characteristics between these two dosage forms and the relative bioavailability of the self-made films was(110.1±10.5)％.
aripiprazoleLC-MS/MSpharmacokineticsorodispersible film
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