Optimization of the Extraction Process and in vitro Anti-Helicobacter pylori Activity of Hezi Qingyou Formula
In this study,based on the observation of the activity of both gallic acid and ellagic acid against Helicobacter pylori(H.pylori)in vitro,an orthogonal test was used to optimize the extraction process of Hezi Qingyou formula,with the adding amount of water,extraction time and extraction times as influencing factors,content of gallic acid and ellagic acid and extraction rate as evaluation indexes.And the in vitro inhibitory activity of the aqueous extracts against H.pylori was investigated.The results showed that the optimized extraction process of Hezi Qingyou formula was as follows:adding 10 times amount of water,extraction 1.5 h per time and 2 times of extraction.Under these conditions,the aqueous extracts of Hezi Qingyou formula showed significant in vitro inhibition of H.pylori growth in a concentration-dependent manner,with the MIC of 80-160 μg/mL.
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