基于高分辨质谱定性分析羌活指纹图谱中的特征峰
Qualitative Analysis on Characteristic Peaks of Notopterygii Rhizoma et Radix in Fingerprints Based on High-resolution Mass Spectrometry
宋金玉 1连云岚 2郑锌 3张禄 2郝云芳 2董培智4
作者信息
- 1. 山西药科职业学院,山西太原 030031
- 2. 山西省检验检测中心药品检验技术研究所,食品药品安全防控山西省重点试验室,山西太原 030031
- 3. 岛津企业管理(中国)有限公司北京分公司,北京 100081
- 4. 山西药科职业学院,山西太原 030031;山西省检验检测中心药品检验技术研究所,食品药品安全防控山西省重点试验室,山西太原 030031
- 折叠
摘要
利用HPLC-高分辨质谱对羌活指纹图谱中的特征峰进行定性.羌活经过甲醇超声提取、离心取上清液,过滤后进样分析.采用电喷雾离子源、正负离子模式;质谱数据采集使用全扫描和数据依赖采集模式,经过LabSolutions Insight软件结合ChemSpider数据库检索,确认了羌活和宽叶羌活指纹图谱中12个特征色谱峰的元素组成及其可能的结构信息,其中1,5-O-二咖啡酰奎宁酸和白花前胡乙素为首次从羌活中鉴定出来,为拓展羌活应用、完善羌活的整体质量评价体系提供了参考.
Abstract
Qualitative analysis was performed on the characteristic peaks in the fingerprint of Notopterygii Rhizoma et Radix by HPLC-high-resolution mass spectrometry.Notopterygii Rhizoma et Radix was extracted by methanol with ultrasound,and then the supernatant was centrifuged and filtered before analysis.The electric spray ion source was used in positive and negative ion mode,and data was collected in full scan and data-dependent acquisition(DDA)modes.Then,these data were retrieved using LabSolutions Insight software combined with the ChemSpider database to confirm the elemental compositions and possible structural information on twelve characteristic chromatographic peaks in Notopterygium incisum Ting ex H.T.Chang and Notopterygium franchetii H.de Boiss.Among them,1,5-O-dicaffeoylquinic acid and praeruptorin B were identified for the first time from Notopterygii Rhizoma et Radix.This study provided a reference for expanding the application of Notopterygii Rhizoma et Radix and improving the overall quality evaluation system of Rhizoma et Radix Notopterygii.
关键词
HPLC/高分辨质谱/羌活/指纹图谱/中药材Key words
HPLC/high-resolution mass spectrometry/Notopterygii Rhizoma et Radix/fingerprint/Chinese medicinal material引用本文复制引用
基金项目
山西省职业院校教师素质提高计划中高职教师素质协同提升项目(2020)(CXTD2020-11)
食品药品安全防控山西省重点实验室资金项目(202204010931006)
出版年
2024
NETL
NSTL
万方数据