基于UPLC-Q-TOF-MS/MS技术分析黑骨藤追风活络胶囊的物质基础及入血成分
Analyses of Material Basis and Components Migrating to Blood of Heiguteng Zhuifeng Huoluo Capsules by UPLC-Q-TOF-MS/MS
田燕荣 1王嘉伟 2李慧馨 3胡晓 2孙宜春4
作者信息
- 1. 贵州中医药大学,贵州贵阳 550025
- 2. 上海医药工业研究院有限公司,上海 201203
- 3. 国药集团同济堂(贵州)制药有限公司,贵州贵阳 550026
- 4. 贵州中医药大学,贵州贵阳 550025;国药集团同济堂(贵州)制药有限公司,贵州贵阳 550026
- 折叠
摘要
采用UPLC-Q-TOF-MS/MS技术对黑骨藤追风活络胶囊提取物的物质基础和入血成分进行了研究,根据化合物的保留时间、准分子离子峰和二级质谱信息,并结合对照品及文献报道,从黑骨藤追风活络胶囊中共鉴定出82个化合物,其中包括生物碱类化合物47个、苯丙素类化合物9个、甾体类化合物7个和其他类化合物19个.在此基础上,在灌胃给药的大鼠血清中共鉴定出9个原型入血成分,包括7个生物碱,即N-去甲基青藤碱、二氢青藤碱、青藤碱、光千金藤碱、木兰花碱、异青藤碱和6-O-去甲基蝙蝠葛波酚碱,以及1个核苷和1个脂肪酸.本研究阐明了黑骨藤追风活络胶囊的物质基础和入血成分,为其后续试验研究提供了参考.
Abstract
The material basis and blood-entering components of the Heiguteng Zhuifeng Huoluo Capsules(HZHC)extract were investigated by ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS).Based on the retention times,quasi-molecular ion peaks,and secondary mass spectrometry information,combined with the reference substances and literature data,a total of 82 compounds were identified from the HZHC,including 47 alkaloids,9 phenylpropanoids,7 steroids,and 19 other compounds.Based on this,9 prototype compounds absorbed into the blood were identified in the serum of rats that received HZHC via gavage,including 7 alkaloids,which were N-demethylsinomenine,dihydrosinomenine,sinomenine,stepharine,magnoflorine,isosinomenine and 6-O-demethylmenisporphine,1 nucleoside and 1 fatty acid.This preliminary investigation elucidated the material basis and blood-absorbed components of the HZHC,which would provide a reference for subsequent experimental research.
关键词
黑骨藤追风活络胶囊/UPLC-Q-TOF-MS/MS/物质基础/入血成分Key words
Heiguteng Zhuifeng Huoluo capsule/UPLC-Q-TOF-MS/MS/material basis/blood-absorbed component引用本文复制引用
基金项目
中央引导地方科技发展专项(GZIM 202001-9-2)
出版年
2024
NETL
NSTL
万方数据