Inhibition of Triptolide Liposomes on Breast Cancer and Its Effect on PI3K/Akt/Cyclin D1 Signaling Pathway
Triptolide liposomes(1-Lips)were prepared by thin film dispersion method,and their physicochemical properties were characterized.The tumor inhibition and the effect of 1-Lips on phosphatidylinositol 3 kinase/protein kinase B/Cyclin D1(PI3K/Akt/Cyclin D1)signaling pathway were investigated with breast cancer-bearing mice.The results showed that 1-Lips had a phospholipid bilayer and a spherical structure with the average particle size of(113.7±2.0)nm,the ξ potential of(-1.1±0.6)mV,and the average encapsulation rate of(90.48±1.24)％.The results of anti-breast cancer effect showed that 1-Lips could significantly reduce the tumor volume,destroy the tissue structure,inhibit the tumor cell proliferation,promote apoptosis,inhibit the expressions of PI3K,p-Akt,Cyclin D1,CDK4,and increase the expression of p21 protein.This demonstrated that 1-Lips could enhance the antitumor effect of 1 and that the antitumor mechanism might be related to the inhibition of the PI3K/Akt/Cyclin D1 signaling pathway.
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