首页|人参蒸晒过程中不同部位化学成分与抗氧化活性的变化研究

人参蒸晒过程中不同部位化学成分与抗氧化活性的变化研究

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为探究九蒸九晒炮制方法对人参不同部位的化学成分和抗氧化活性的影响,该试验采用超高效液相色谱法测定在九蒸九晒炮制过程中人参不同部位的10种人参皂苷和5-羟甲基糠醛(5-HMF)的含量,并测定其抗氧化活性,结合主成分分析(PCA)、聚类分析(HCA)和正交偏最小二乘判别分析法(OPLS-DA)进行质量评价.结果显示,人参皂苷Rg1、Re、Rb1、Rd、Rc、Rb2的含量先升高后降低;人参皂苷Rg3、Rk1、Rg5、Rh1的含量逐渐升高;5-HMF的含量先升高后降低.PCA结果共提取到2个主成分,HCA结果将须根部位与其他部位进行区分,OPLS-DA结果确定了人参皂苷Rg5、Rk1,、Rg1,和Re、Rg3、Rh1为主要差异性成分.不同蒸晒次数的人参的抗氧化活性具有一定差异,其中,人参第二次蒸晒的须根样品对羟自由基清除能力最强.本研究通过九蒸九晒的炮制和化学计量学模式的识别,揭示了人参不同根部部位化学成分和抗氧化活性之间的规律,即稀有人参皂苷与抗氧化活性呈现负相关性.此结果可为人参的进一步开发利用提供科学依据.
Study on the Changes of Chemical Composition and Antioxidant Activity in Different Parts of Ginseng during Steaming and Drying
In order to investigate the effects of the processing method on the chemical composition and antioxidant activity of different parts of Ginseng,the contents of 10 kinds of ginsenosides and 5-hydroxymethylfurfural(5-HMF)of different parts of Ginseng during the processing were determined by ultra-high performance liquid chromatography,and their antioxidant activities were determined.Principal component analysis(PCA),cluster analysis(HCA)and orthogonal partial least squares discriminant analysis(OPLS-DA)were combined to evaluate the quality.The results showed that the contents of ginsenosides Rg1,Re,Rb1,Rd,Rc and Rb2 increased first and then decreased,while the contents of ginsenosides Rg3,Rk1,Rg5 and Rh,increased gradually.The content of 5-HMF increased first and then decreased.Two principal components were extracted by PCA,the fibrous root parts were distinguished from other parts by HCA,and ginsenosides Rg5,Rk1,Rg1+Re,Rg3 and Rh1 were identified by OPLS-DA as the main difference components.The antioxidant activity of Ginseng varied with different steaming times.Among them,Ginseng fibrous root samples steamed and sun-cured for the second time had the strongest scavenging ability of hydroxyl free radicals.This study revealed the relationship between chemical components and antioxidant activities in different parts of Ginseng root through the processing method named"nine steaming and nine drying"and the stoichiometric pattern recognition,that is,rare ginsenosides showed a negative correlation with antioxidant activity,which provided a scientific basis for further development and utilization of Ginseng.

Ginsengnine steaming and nine dryingdifferent parts of the rootchemometricsantioxidant

姜芯、李茗晞、王艳、郭若曦、李雪

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天津中医药大学中药制药工程学院,天津 301617

天津市中药智能制药与绿色制药重点实验室,天津 301617

现代中医药海河实验室,天津 301617

天津尚美化妆品有限公司,天津 300385

天津医科大学总医院核医学科,天津 300052

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人参 九蒸九晒 根部的不同部位 化学计量学 抗氧化

国家自然科学基金项目现代中医药海河实验室科技计划项目—具有皮肤靶向性的人参祛皱抗衰面霜开发、天津市技术创新引导专项(基金)企业科技特派员项目中国工程院项目—中医药产业高质量发展战略研究项目

8160277323YDTPJC005902023-XBZD-11-01

2024

10.16522/j.cnki.cjph.2024.07.013

中国医药工业杂志
上海医药工业研究院,中国化学制药工业协会

中国医药工业杂志

CSTPCD
影响因子：0.487
ISSN：1001-8255
年,卷(期)：2024.55(7)