Comparison between a New Type of Cell Intelligent Manufacturing Line and Manual Preparation
To address the shortcomings of low efficiency,high cost and large batch-to-batch variation of manually prepared cells,this study developed a novel smart production line for human umbilical cord mesenchymal stem cells(hUC-MSCs),and compared the basic cell characteristics,safety and efficacy of the obtained cells with those of traditional manually prepared cells.The results showed that the hUC-MSCs prepared by the smart production line were fibrous,and the cell surface markers met the requirements of MSCs defined by the International Society for Cell & Gene Therapy.The results of cell sterility and mycoplasma were negative,and the content of endotoxin was lower than 0.5 EU/mL.The results of oncogenes,oncogenes suppressor and karyotyping analyses were normal.The expression of telomerase was in low activity.The results of abnormal toxicity were normal.The cell viability was higher than 90％.And the strong capabilities of osteogenesis,adipogenesis and chondrogenesis were observed under the microscope.And the cells could inhibit the proliferation of Th1,Th2 and Th17 cells and promote the proliferation of Treg cells.The hUC-MSCs prepared by this production line met the quality standards and showed no significant difference compared with those prepared manually.In conclusion,the cell intelligent production line developed in this study is more advantageous in terms of cell homogeneity,capacity enhancement,cost optimization and superiority of deployment,which provides a new idea to realize the scale-up and industrialization of cell preparation.
万方数据
维普
