Determination of Salbutamol Sulfate in Rat Respiratory Tract by LC-MS/MS and Its Lung Tissue Distribution
The LC-MS/MS method was established to determine salbutamol sulfate in rat tracheobronchial and lung tissues.The results showed that the linear ranges in tracheobronchial and lung tissues were 10.41-10 410 and 5.205-5 205 ng/g,respectively.The intra-and inter-day RSDs of the samples from different tissues were less than 15%.After inhalation administration,salbutamol sulfate showed similar dynamic changes in the tracheobronchial and lung tissues of rats,and reached maximum concentration immediately after administration,followed by rapid elimination.The inhalation of salbutamol sulfate solution was widely distributed in the tracheal and bronchus of the target site,which provided a reference basis for the clinical use.
salbutamol sulfateatomization inhalationLC-MS/MStracheal and bronchuslung tissue