Several nanoemulsions with different contents of ω-3 polyunsaturated fatty acid glyceride were prepared by the high-speed shearing and high-pressure homogenization method and their effects on the treatment of liver injury were investigated.The combination of optimized process parameters selected through the experiment of single factor was as follows:the oil phase and the water phase were sheared at a shearing speed of 8 000 r/min for 5 min at 60 ℃,and then homogenized at a pressure of 60 000 kPa for 6 cycles.The nanoemulsion prepared with the optimized process parameters presented a white emulsion appearance,with an average particle size of approximately 200 nm and a pH value of approximately 7.4,and all indicators met the quality requirements.The results of the cell uptake test showed that the prepared nanoemulsions could be effectively taken up by human fetal hepatocyte cell line(LO2 cell),and the uptake amount was positively correlated with the ω-3 polyunsaturated fatty acid content.As for the cholestatic liver injury model mouse,tail vein injection of nanoemulsions could effectively improve liver injury,and the nanoemulsions with a high ω-3 polyunsaturated fatty acid glyceride content showed a better therapeutic effect on liver-injured mice.In conclusion,the co-3 polyunsaturated fatty acid glyceride nanoemulsions prepared in this study had a significant improvement effect on liver injury and good clinical application potential,providing an effective reference for the optimal design of injectable glyceride nanoemulsion drugs.
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