Analysis on Metabolites and Metabolic Pathways of Lycii Fructus in Rats
The ultra-high performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS)method was established to study the prototypical components and metabolites of Lycii Fructus extract in serum,urine,faeces,and bile of rats and to clarify their metabolic processes.The Waters ACQUITY UPLC BEH C18 column(2.1 mm×50 mm,1.7 pm)was used,and the analysis was carried out in the gradient elution mode with acetonitrile as mobile phase A,and 0.1％formic acid as mobile phase B.The column temperature was 30 ℃,the flow rate was 0.3 mL/min,and the injection volume was 3 pL.The results showed that a total of 46 chemical components were identified from various biological samples,including 17 prototypical components and 29 metabolites.Its metabolic pathways included phase Ⅰ metabolic reactions(hydroxylation,dehydroxylation,demethylation,hydrolysis,and reduction)and phase Ⅱ metabolic reactions(methylation,glucuronidation,sulfation,and sulfonation).This study preliminarily elucidated the metabolic process of Lycii Fructus in vivo,and laid a foundation for the subsequent elucidation of its pharmacological material basis and development.
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