Establishment and Validation of a Rapid Method for Detecting Uricase Activity in Serum of Human
Based on the principle that uricase catalyzes the decomposition of uric acid to produce allantoin,hydrogen peroxide and carbon dioxide,this paper established a method to detect the activity of uricase at 37 ℃ and pH 7.5.The results showed that the quantitative ranges of hydrogen peroxide and uricase were 0.049-100.000 μmol/L and 0.819-200.000 mU/mL,respectively.The within-and between-run variation coefficients of precision were less than 20.0％,and the relative error of accuracy were within±25.0％.The results of recovery experiments using hyperlipemia samples(lipid index 3 mg/mL)and 2％hemolysis donor samples compared to normal donor samples showed that hyperlipemia did not interfere with the detection of uricase activity,whereas hemolysis did.Uricase activity was not affected when human serum uricase samples were placed at room temperature or 2-8 ℃ for no more than 24 h,frozen and thawing for no more than 3 cycles,and stored at-70-90 ℃ for no more than 64 days.In conclusion,the established method is sensitive,precise,accurate,simple,rapid,and can support the evaluation of preclinical and clinical studies of uricase drugs.
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