Exploration of constructing a rabbit knee osteoarthritis model using the modified Videman method
Objective To establish a rabbit knee osteoarthritis(KOA)model that is easy to operate,stable,efficient,and convenient for treatment and efficacy observation.Methods A total of 12 healthy male New Zealand rabbits were divided into a control group and a model group using the random number table method,with 6 rabbits in each group.The medical splint was used to fix the right knee joint of the rabbit in an extended position,with each fixation lasting for 60 minutes,twice a day,and lasting for 4 weeks for modeling.The Lequesne MG scoring method was used to assess the motor ability of the rabbit's right knee joint,the general morphology of the rabbit's right knee joint was observed and recorded,safranine O staining was used to determine cartilage damage,and the modified Mankin's joint cartilage pathology scoring method was used to compare and analyze the two groups of rabbits.Results The comprehensive Lequesne MG score of the model group was(2.00[0.00,8.00])points,which was higher than that of the control group,with statistically significant difference(P<0.05).The general morphology of the knee joint showed that compared with the control group,the model group had swollen knee joints,narrowed joint cavities,worn cartilage,and rough surface texture.The results of safranine O staining showed that compared with the control group,the model group had a disordered cartilage tissue structure,reduced matrix staining,incomplete tidal line,uneven surface of cartilage,and a diverse and irregular arrangement of chondrocytes.In terms of the modified Mankin's joint cartilage pathology score,the comprehensive score of the model group was(3.00[0.00,7.50])points,higher than that of the control group,with statistically significant difference(P<0.05).Conclusion The modified Videman method for constructing a rabbit KOA model has the advantages of simple operation,firm fixation,and high success rate,which is conducive to the study of the mechanism of KOA occurrence and development.
万方数据
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