Objective To establish a quantitative analysis of multi-components by single-marker for simultaneous determination of contents of β-asarone and α-asarone in Acorus tatarinowii.Methods The chromatographic column Agilent Eclipse XDB C18(5 μm,4.6 mm×150 mm)was selected.Mobile phase:acetonitrile-0.05％phosphoric acid water for gradient elution.α-asarone was used as the internal standard,the relative correction factor of β-asarone was calculated and its content was determined.Results The R2 values of β-asarone and α-asarone were both 0.9999,indicating a good linear relationship between the injection concentrations of the two components within the corresponding range.The average injection recovery rates of β-asarone and α-asarone were 98.69％and 100.24％,respectively,with RSD of 2.04％and 2.06％,respectively.There was no significant difference between the results obtained by the quantitative analysis of multi-components by single marker and the external standard method.Conclusion This method is rapid,simple,reproducible,and feasible,and can be used for the quality control of Acorus tatarinowii.
关键词
石菖蒲/一测多评法/β-细辛醚/α-细辛醚
Key words
Acorus tatarinowii/Quantitative analysis of multi-components by single marker/β-asarone/α-asarone
维普
万方数据