Establishment of an HPLC-RID method for determining the content of the Lycium barbarum polysaccharides in Fructus lycii
Objective To explore the extraction method and content determination method of Lycium barbarum polysaccharides(Lycium barbarum polysaccharides measured by glucose)in Fructus lycii through high-performance liquid chromatography-refractive index detector(HPLC-RlD),to achieve the purpose of accurate,reliable,and easy operation of the detection method.Methods This method adopted the method of adding water for reflux extraction and was detected by high-performance liquid chromatography and differential refractive index detector.The chromatographic column was LC026(Prevail Carbohyrate ES as filler with 250 mm×4.6 mm,5 μm),the mobile phase was acetonitrile-water(70:30),and the detector temperature was 40℃.The flow rate was 1.0 ml/min,and the column temperature was 40℃.Results There was a good linear relationship between Fructus lycii in the linear range of 0.5-4.0 mg/ml,with R2 = 0.9999,an average recovery rate of 104.81％,and RSD=1.10％.Conclusion This method is simple to operate,highly sensitive,reproducible,accurate,and reliable,and can be used to determine the content of Lycium barbarum polysaccharides in the medicinal Fructus lycii,and effectively control the quality of Fructus lycii.
Fructus lyciiLycium barbarum polysaccharidesDifferential refractive index detectorHigh-performance liquid chromatography
万方数据
维普
