HPLC法测定蒙药沙蓬茎、叶、花中槲皮素和异鼠李素的含量
Determination of quercetin and isorhamnetin in the stems,leaves,and flowers of Mongolian medicine Agriophyllum squarrosum by HPLC
乌雅汉 1莫日根 2呼和 1额尔敦呼 3乌日汗 1白明君 1包书茵 1包桂花1
作者信息
- 1. 内蒙古民族大学,内蒙古通辽 028000
- 2. 内蒙古呼伦贝尔市新巴尔虎右旗阿拉坦额莫勒镇社区卫生服务中心,内蒙古阿拉坦额莫勒镇 021300
- 3. 内蒙古自治区国际蒙医医院国家蒙药制剂中心,内蒙古呼和浩特 010020
- 折叠
摘要
目的 建立蒙药沙蓬茎、叶、花中槲皮素和异鼠李素的含量,分析槲皮素和异鼠李素在沙蓬不同部位的分布情况.方法 采用高效液相色谱法(HPLC)测定槲皮素和异鼠李素的含量.结果 槲皮素和异鼠李素分别在 0.0107～0.5336 μg、0.0421～2.1038 μg范围内与峰面积具有很好的线性关系,回收率分别为100.06%、102.59%,相对标准偏差(RSD)分别为 1.52%、1.67%(n=6),沙蓬茎、叶、花中槲皮素和异鼠李素的总量依次为叶>花>茎.结论 该方法精密度高,分离度好,可用于蒙药沙蓬茎、叶、花中槲皮素和异鼠李素的含量测定.
Abstract
Objective To determine the content of quercetin and isorhamnetin in the stems,leaves,and flowers of Mongolian medicine Agriophyllum squarrosum,and analyze the distribution of quercetin and isorhamnetin in different parts of Agriophyllum squarrosum.Methods High-performance liquid chromatography(HPLC)was used to determine the content of quercetin and isorhamnetin.Results Quercetin and isorhamnetin had a good linear relationship with the peak area within the range from 0.0107 to 0.5336 μg,0.0421 to 2.1038 μg,respectively,with recovery rates of 100.06%and 102.59%,and relative standard deviation(RSD)of 1.52%and 1.67%,respectively(n=6).The total content of quercetin and isorhamnetin in the stems,leaves,and flowers of Agriophyllum squarrosum is in the order of leaves>flowers>stems.Conclusion This method has high precision and good separation,and can be used for content determination of quercetin and isorhamnetin in the stems,leaves,and flowers of Mongolian medicine Agriophyllum squarrosum.
关键词
蒙药/沙蓬/茎、叶、花/异鼠李素/槲皮素/高效液相色谱法Key words
Mongolian medicine/Agriophyllum squarrosum/Stems,leaves,and flowers/Isorhamnetin/Quercetin/High-performance liquid chromatography引用本文复制引用
基金项目
国家自然科学基金(82004070)
内蒙古自治区高等学校青年科技人才发展项目(NJYT23134)
内蒙古自治区"草原英才"工程青年创新人才项目()
内蒙古自治区科技计划(2020GG0103)
内蒙古自治区蒙医药标准化项目(2023-MB030)
内蒙古自治区自然科学基金(2023LHMS08047)
内蒙古自治区科技重大专项(2019)(2019ZD004)
出版年
2024
国家科技期刊平台
NSTL
万方数据