Response difference of PD-1 inhibitor combined with Aitan in triple negative breast cancer mouse model
Objective To explore the response difference of programmed death receptor 1(PD-1)inhibitor combined with Aitan(Apatinib)in a triple negative breast cancer(TNBC)mouse model.Methods 4T1 cells were used to establish a TNBC mouse model,and 32 mice were randomly divided into the model group,the PD-1 inhibitor group,the Aitan group and the combination treatment group after tumor formation,with 8 mice in each group.The tumor volume and tumor inhibition rate of each group of mice were recorded and calculated,and the expression of programmed death ligand 1(PD-L1)in serum and tumor cells was detected.Results At 7 d,14 d,21 d,and 28 d after modeling,the tumor volume of the model group was significantly higher than that of the PD-1 inhibitor group,Aitan group,and combination treatment group(P<0.05).The tumor volume of the model group increased rapidly 7-14 days after modeling,while the tumor volume of other groups was significantly inhibited.The tumor size of the combination treatment group was significantly smaller than that of the PD-1 inhibitor group and the Aitan group(P<0.05).After 28 days of modeling,the tumor inhibition rate of the combination treatment group was significantly higher than that of the PD-1 inhibitor group and the Aitan group,with statistically significant difference(P<0.05).The serum PD-L1 and tumor positivity rates in the PD-1 inhibitor group and the combination treatment group were significantly higher than those in the model group and the Aitan group,with statistically significant differences(P<0.05).There were no significant differences in the positive rates of serum PD-L1 and tumor PD-L1 between the PD-1 inhibitor group and the combination treatment group(P>0.05).Conclusion The combination of PD-1 inhibitors and Aitan has a synergistic anti-tumor effect in the treatment of TNBC,which can effectively inhibit tumor growth.
Programmed death ligand 1Triple negative breast cancerAitanMouse
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