Study on the effect of Sheshang Capsule on platelet activation function in rabbits injured by Trimeresurus stejnegeri
Objective To study the effect of Sheshang Capsule on platelet activation function in rabbits injured by Trimeresurus stejnegeri.Methods A total of 24 New Zealand white rabbits were randomly divided into a normal control group,a model group,and a Sheshang Capsule group,with 8 rabbits in each group,half male and half female.According to the previous research method,the model group and the Sheshang Capsule group were injected with 0.75 mg/kg Trimeresurus stejnegeri's venom into the rabbit ear vein to establish a rabbit injured by Trimeresurus stejnegeri model,while the normal control group was injected with an equal amount of physiological saline.After 6 hours of injection,the normal control group and the model group were gavaged with 10 ml/(kg·d)physiological saline,while the Sheshang Capsule group was gavaged with 10 ml/(kg·d)solution with Sheshang Capsule content.After continuous gavage of the above three groups for one week,5 ml of blood was collected from the rabbit ear vein respectively.After platelet separation and extraction,real-time fluorescence quantitative polymerase chain reaction(qPCR)was used to detect platelet activation indicators,i.e.,integrin αⅡb(CD41),integrin βⅢ chain(CD61),platelet P-selectin(CD62P),and lysosomal membrane glycoprotein(CD63)messenger RNA(mRNA)expression,and enzyme-linked immunosorbent assay(ELISA)to detect membrane glycoprotein Ⅱb/Ⅲa(GPⅡb/Ⅲa)levels in each group.The differences in platelet activation indicators CD41,CD61,CD62P,CD63mRNA expression,and GPⅡb/Ⅲa levels was analyzed and compared among different groups.Results There were significant differences in CD41,CD61,CD62P,CD63 mRNA expression and GP Ⅱ b/Ⅲ a levels between groups(P<0.01).Compared with the normal control group,the expression of CD41,CD61,CD62P,CD63 mRNA,and GP Ⅱ b/Ⅲ a levels in the model group were significantly reduced,with the differences were statistically significant(P<0.01),while the expression of CD41,CD61,CD62P and CD63 mRNA in the Sheshang Capsule group was significantly reduced,with the differences were statistically significant(P<0.01),and GPⅡb/Ⅲa levels in the Sheshang Capsule group were significantly reduced,with the differences were statistically significant(P<0.05).Compared with the model group,the expression of CD41,CD61,CD62P,and CD63 mRNA in the Sheshang Capsule group was significantly increased,with the differences were statistically significant(P<0.01),and the level of GPⅡb/Ⅲa in the Sheshang Capsule group was significantly increased,with the differences were statistically significant(P<0.05).Conclusion When injured by Trimeresurus stejnegeri,the platelet activation function is inhibited.The Sheshang Capsule can reduce the inhibition of platelet activation function and improve platelet activation function by increasing the levels of CD41,CD61,CD62P,CD63,and GPⅡb/Ⅲa.
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