Effect of Shentong Zhuyu Keli on migraine rats and its influence on MAPK signal pathway
Objective To investigate the analgesic effect of Shentong Zhuyu Keli(SZK)on migraine rats and its influence on mitogen-activated protein kinase(MAPK)signaling pathway.Methods A total of 60 SD rats were randomly divided into the control group,the model group,the low-,medium-and high-dose SZK groups and the carbamazepine group.Except for the control group,the migraine rat model was established by penicillin G potassium for all groups.The effect of SZK on the pain latency time,the number of pain reactions and the pain duration of migraine rats was observed.the levels of 5-hydroxy ryptamine(5-HT),norepinephrine(NE),dopamine(DA)and endothelin-1(ET-1)in brain tissue were measured by ELISA.Western blot was adopted to detect the protein expression levels of extracellular signal regulated-kinase(ERK),phosphorylated extracellular regulatory protein kinase(p-ERK),P38 mitogen-activated protein kinase(P38MAPK),phosphorylated P38 mitogen-activated protein kinase(p-P38MAPK),c-Jun N-terminal kinase(JNK),phosphorylated c-Jun N-terminal kinase(p-JNK),and glyceraldehyde-3-phosphate dehydrogenase(GAPDH).Results Compared with the model group,the pain latency time in the SZK groups and the carbamazepine group was significantly prolonged,the number of spontaneous and induced pain decreased significantly,and the duration of spontaneous and induced pain was significantly shortened,with statistically significant differences(P＜0.05).compared with the model group,the duration of spontaneous pain in the high-dose SZK group and the carbamazepine group was significantly shortened,with statistically significant difference(P＜0.05).Compared with the model group,the levels of 5-HT,NE and DA in the low-,medium-and high-dose SZK groups and the carbamazepine group were significantly higher,and the level of ET-1 was significantly lower,with statistically significant differences(P＜0.05).Compared with the model group,the expression levels of p-ERK,p-JNK,p-P38MAPK and P38MAPK in the brain tissue of migraine rats in the middle-and high-dose SZK groups increased significantly,the expression levels of p-JNK and P38MAPK in the low-dose SZK group increased significantly,and the expression level of P38MAPK in the carbamazepine group increased significantly,with statistically significant differences(P＜0.05).Conclusion SZK can inhibit the migraine symptoms induced by penicillin G potassium in rats by increasing the protein expression levels of p-JNK,p-ERK,p-P38MAPK and P38MAPK in MAPK signaling pathway.
Shentong Zhuyu KeliMigraine5-hydroxytryptamineNorepinephrineDopamineMitogen-activated protein kinase pathway
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