摘要
目的 利用生物信息学分析溶质运载体16A(SLC16A)基因家族在低级别胶质瘤(LGG)发生发展中的作用及预后价值.方法 使用ONCOMINE、GEPIA、HPA、TCGA、LinkedOmics、GSEA、TIMER等公共数据库研究SLC16A基因家族在LGG中的mRNA表达谱,筛选出差异性表达并影响LGG预后的基因,全面分析其在LGG发生发展中的作用机制、诊断和预后价值.结果 LGG中SLC16A1、SLC16A3、SLC16A4表达水平高于正常脑组织,且随着肿瘤分期的增长而上升,高表达组总生存期(OS)和无病生存期(DFS)较短.LGG细胞质和细胞膜中可见大量SLC16A1、SLC16A3和SLC16A4蛋白表达.这三种基因与炎症反应、免疫细胞浸润显著相关.结论 SLC16A1、SLC16A3和SLC16A4可作为LGG的潜在诊断和预后生物标志物,并通过免疫调控和炎性微环境参与LGG的发生发展.
Abstract
Objective To analyze the role and prognostic value of the solute carrier 16A(SLC16A)gene family in the occurrence and development of low-grade gliomas(LGG)using bioinformatics.Methods Public databases such as ONCOMINE,GEPIA,HPA,TCGA,LinkedOmics,GSEA,TIMER,etc.,were used to study the mRNA expression profile of the SLC16A gene family in LGG,screen for differentially expressed genes that affect the prognosis of LGG,and comprehensively analyze their mechanism of action,diagnostic value and prognostic value in the occurrence and development of LGG.Results The expression levels of SLC16A1,SLC16A3,and SLC16A4 in LGG were higher than those in normal brain tissue and increased with the growth of the tumor stage.The high-expression group had shorter overall survival(OS)and disease-free survival(DFS).Large amounts of SLC16A1,SLC16A3,and SLC16A4 protein expression could be observed in the cytoplasm and cell membrane of LGG.These three genes were significantly associated with inflammatory response and immune cell infiltration.Conclusion SLC16A1,SLC16A3,and SLC16A4 can serve as potential diagnostic and prognostic biomarkers for LGG,and participate in the occurrence and development of LGG through immune regulation and control and inflammatory microenvironment.
基金项目
江苏省南通市卫生健康委科研课题(QN2023009)
维普
万方数据