Optimization of modeling method of rat model of oral submucous fibrosis
Objective To compare three methods of establishing rat model of oral submucous fibrosis(OSF):arecoline rubbing,buccal submucosal injection of arecoline,arecoline rubbing combined with buccal submucosal injection of arecoline,so as to explore the better method of establishing rat OSF model.Methods Forty male SPF grade SD rats were randomly divided into the blank control group,the arecoline rubbing group,the arecoline injection group and the arecoline rubbing plus injection group,with 10 rats in each group.The living conditions,mouth opening and weight changes of rats in each group were observed every week after treatment.After 8 weeks of treatment,rats were sacrificed.The buccal mucosa was taken and stained with hematoxylin-eosin(HE)to observe the inflammatory changes of buccal mucosa,the positive degree of transforming growth factor-beta1(TGF-β1)in buccal mucosa was detected by immunohistochemistry,and the expression of TGF-β1 protein in buccal mucosa was detected by western blot.Results After 8 weeks of modeling,the buccal mucosa of each group showed varying degrees of whitening and fiber-like changes.Thereinto,the degree of submucosal fibrosis in both cheeks of rats in the arecoline rubbing group was different and uneven.There was no significant difference in body weight between the arecoline rubbing group and blank control group(P>0.05),but the weight of rats in the arecoline injection group and the arecoline rubbing plus injection group all decreased(P<0.05),and the weight of rats in arecoline rubbing plus injection group decreased most obviously(P<0.05).Compared with the blank control group,the mouth opening of OSF rats in the three models decreased(P<0.05),and the mouth opening of the arecoline injection group and the arecoline rubbing plus injection group decreased more obviously(P<0.05).Compared with the blank control group,the expression levels of TGF-β1 in OSF rats with three models were significantly increased(P<0.05),the differences among the model groups were also statistically significant(P<0.05),and the expression level of TGF-β1 was the highest in the arecoline rubbing plus injection group.Conclusion Arecoline rubbing combined with buccal submucosal injection of arecoline is the best method to establish oral submucous fibrosis model in rats.
万方数据
维普
