Exploring the protection and mechanism of broad chest aerosol on the heart of blood stasis type rats based on the Nrf2/ARE signal pathway
Objective To elucidate the protective effect and possible mechanism of Kuanxiong Aerosol(KA)on myocardial ischemia reperfusion injury(MIRI)in rats.Methods Thirty healthy male rats were randomly divided into a sham surgery group(SHAM)group,a MIRI group,and a MIRI+KA group.After 28 days of administration(physiological saline),a MIRI model was constructed,and a mouse MIRI model was established by ligating the coronary artery.After surgery,the MIRI+KA group continued to receive KA intervention,with one press each time and twice a day.The SHAM group and MIRI groups were given physiological saline once a day.Collect mouse myocardial tissue after surgery and weigh the mouse heart.HE staining was used to observe histopathological changes in the tissue;Western blot was used to detect the expression of oxidative stress and apoptosis related proteins.Results The MIRI group mice showed significant infiltration of inflammatory cells in the myocardial interstitium,increased expression of oxidative stress protein MDA,and decreased expression of SOD and CAT proteins(P<0.05).The treatment with KA can significantly reduce the heart weight and heart weight index induced by MIRI in mice,reduce the infiltration of inflammatory cells in the myocardial interstitium,and improve the expression of SOD protein.In addition,treatment with KA can significantly increase the expression of nuclear factor erythroid-2 related factor 2(Nrf2)protein and reduce the expression of antioxident response element(ARE)protein(P<0.05).Conclusion KA has protective effect on myocardial ischemia-reperfusion injury in mice,and its protective mechanism may be related to the activation of Nrf2/ARE signaling pathway.
万方数据
维普
