Influence of Lanzhu Huazhuo Decoction on lipid metabolism and oxidative stress caused by impaired glucose regulation
Objective To investigate the influence of Lanzhu Huazhuo Decoction on lipid metabolism and oxidative stress caused by impaired glucose regulation.Methods A total of 30 in the 36 SD male rats were used to establish rat models with impaired glucose regulation.After successful modeling,the 30 rats were randomly divided into the model group(n=6),the metformin group(n=6,200 mg/kg),the low-dose Lanzhu Huazhuo Decoction group(n=6,5 g/kg),the middle-dose Lanzhu Huazhuo Decoction group(n=6,10 g/kg)and the high-dose Lanzhu Huazhuo Decoction group(n=6,20 g/kg).The other 6 rats were set as the blank group.The treatment groups were administered at different doses by gavage,while the model group and the blank group were administered with 1 ml/100 g physiological saline once a day for 4 consecutive weeks.Fasting blood glucose(FBG)and oral glucose tolerance test 2 h plasma glucose(OGTT2hPG)were measured by tail tip blood sampling,and the body weight of rats in each group was measured.Serum levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C)and high-density lipoprotein cholesterol(HDL-C)were analyzed biochemically,and serum levels of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were detected using a reagent kit.Results Compared with the blank group,the body weight of rats,and the levels of HDL-C,SOD and GSH-Px in the model group decreased,while the levels of FBG,OGTT2hPG,TC,TG,LDL-C and MDA increased,with statistically significant differences(P<0.05).Compared with the model group,the body weight of the rats,and the levels of HDL-C,SOD and GSH-Px in the middle-dose and high-dose groups of Lanzhu Huazhuo Decoction increased,while the levels of FBG,OGTT2hPG,TC,TG,LDL-C and MDA decreased,with statistically significant differences(P<0.05).Conclusion Lanzhu Huazhuo Decoction can effectively regulate lipid metabolism and oxidative stress,thus improving impaired glucose regulation.
万方数据
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