摘要
目的 探讨兰术化浊汤对糖调节受损脂代谢及氧化应激的影响.方法 36只SD雄性大鼠,将其中30只构建糖调节受损大鼠模型,造模成功后,30 只大鼠随机分为模型组、二甲双胍组(200 mg/kg)、兰术化浊汤低剂量组(5 g/kg)、兰术化浊汤中剂量组(10 g/kg)、兰术化浊汤高剂量组(20 g/kg),每组各 6 只,另 6 只设为空白组.治疗组分别按不同剂量灌胃给药,模型组与空白组按 1 ml/100 g生理盐水灌胃,1 次/d,连续 4 周.通过尾尖采血的方式检测空腹血糖(FBG)、糖耐量试验 2 h血糖(OGTT2hPG),测量各组大鼠体重,生化分析血清总胆固醇(TC)、三酰甘油(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)水平,试剂盒检测血清丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)水平.结果 与空白组比较,模型组大鼠体重、HDL-C、SOD、GSH-PX水平降低,FBG、OGTT2hPG、TC、TG、LDL-C、MDA水平升高,差异有统计学意义(P<0.05);与模型组比较,兰术化浊汤中、高剂量组大鼠体重、HDL-C、SOD、GSH-PX水平升高,FBG、OGTT2hPG、TC、TG、LDL-C、MDA水平降低,差异有统计学意义(P<0.05).结论 兰术化浊汤可有效调节脂质代谢和氧化应激,从而改善糖调节受损.
Abstract
Objective To investigate the influence of Lanzhu Huazhuo Decoction on lipid metabolism and oxidative stress caused by impaired glucose regulation.Methods A total of 30 in the 36 SD male rats were used to establish rat models with impaired glucose regulation.After successful modeling,the 30 rats were randomly divided into the model group(n=6),the metformin group(n=6,200 mg/kg),the low-dose Lanzhu Huazhuo Decoction group(n=6,5 g/kg),the middle-dose Lanzhu Huazhuo Decoction group(n=6,10 g/kg)and the high-dose Lanzhu Huazhuo Decoction group(n=6,20 g/kg).The other 6 rats were set as the blank group.The treatment groups were administered at different doses by gavage,while the model group and the blank group were administered with 1 ml/100 g physiological saline once a day for 4 consecutive weeks.Fasting blood glucose(FBG)and oral glucose tolerance test 2 h plasma glucose(OGTT2hPG)were measured by tail tip blood sampling,and the body weight of rats in each group was measured.Serum levels of total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C)and high-density lipoprotein cholesterol(HDL-C)were analyzed biochemically,and serum levels of malondialdehyde(MDA),superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)were detected using a reagent kit.Results Compared with the blank group,the body weight of rats,and the levels of HDL-C,SOD and GSH-Px in the model group decreased,while the levels of FBG,OGTT2hPG,TC,TG,LDL-C and MDA increased,with statistically significant differences(P<0.05).Compared with the model group,the body weight of the rats,and the levels of HDL-C,SOD and GSH-Px in the middle-dose and high-dose groups of Lanzhu Huazhuo Decoction increased,while the levels of FBG,OGTT2hPG,TC,TG,LDL-C and MDA decreased,with statistically significant differences(P<0.05).Conclusion Lanzhu Huazhuo Decoction can effectively regulate lipid metabolism and oxidative stress,thus improving impaired glucose regulation.
维普
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