Simultaneous determination of aflatoxin,zearalenone,and ochratoxin A in Massa Medicata Fermentata by high-performance liquid chromatography and fluorescence detection
Objective To establish a method for the simultaneous detection of aflatoxin,zearalenone,and ochratoxin A in Massa Medicata Fermentata using immunoaffinity column purification-post column photochemical derivatization-high-performance liquid chromatography.Methods The sample was extracted with 60%acetonitrile ultrasound,purified with an immunoaffinity column,and treated for gradient elution with XBridge®Phenyl column(4.6 mm×250 mm,5 μm),and with acetonitrile and 0.1%phosphoric acid solution as the mobile phase,derivatization with a photochemical derivation instrument,and detection by switching fluorescence wavelength.Results The linear ranges of the standard curves for aflatoxin,zearalenone,and ochratoxin A were 0.006-0.108,0.500-2.500,and 0.202-1.009 ng,respectively.The linear relationships of the six toxins were above 0.9994,with detection limits of 1.2,100.0,and 40.3 ng/ml,and an average spiked recovery rate of 73.2%-92.3%.The relative deviation was 2.1%-5.4%.Conclusion This method has the characteristics of strong specificity and easy operation,and can be effectively used for the simultaneous detection and safety quality control of three toxins in Massa Medicata Fermentata.
Massa Medicata FermentataAflatoxinZearalenoneOchratoxin AHigh-performance liquid chromatography and fluorescence detection
万方数据
维普
