Impacts of different concentrations of etomidate on the biological function of colorectal cancer cells by regulating the expression of FEZF1-AS1
Objective To investigate the impacts of different concentrations of etomidate on the biological function of colorectal cancer cells,so as to analyze its potential mechanism.Methods 0,4,16 and 64 μmol/L etomidate were used to treat colorectal cancer cells(LS513 cells)for 48 hours,the cell survival rate and apoptosis rate were determined,and the expression levels of Bcl-2,BAX,Cleaved caspase-3,LncRNA FEZF1-AS1 and miR-363-3p were detected.The cultured LS513 cells were divided into the blank group,the 64 μmol/L etomidate group and the pcDNA-FEZF1-AS1 group.The biological functions of the three groups of cells were compared.Results After treating LS513 cells with etomidate at 4,16,64 μmol/L,the cell survival rate,the number of migrating cells and the number of invading cells all decreased,the apoptosis rate increased,and the expression of apoptosis-related proteins Cleaved caspase-3 and Bax increased,while the expression of Bcl-2 decreased(P<0.05).After overexpression of LncRNA FEZF1-AS1 in LS513 cells,the expression of LncRNA FEZF1-AS1 increased,and the expression of miR-363-3p decreased.In addition,after overexpression of LncRNA FEZF1-AS1,the survival rate,the number of migrating cells and the number of invading cells increased and the apoptosis rate decreased(P<0.05).Conclusion Etomidate can inhibit the migration and invasion of colorectal cancer cells,and promote their apoptosis,and may play a role by regulating LncRNA FEZF1-AS1/miR-363-3p signal axis.
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