Study on the role of long-chain noncoding RNA H19 in the pathogenesis of ulcerative colitis-related colorectal cancer in mice
Objective To explore the expression and significance of long-chain noncoding RNA H19(H19)in the pathogenesis of ulcerative colitis-associated colorectal cancer(CAC)in mice.Methods A total of thirty C57BL/6 mice were randomly divided into the control group and the observation group.The model induced by azoxymethane and dextran sodium sulfate was established in the mice of the observation group.At the same time,mice in the control group were given intraperitoneal injection of sterile saline and were allowed to drink clean water.On the 71st day,the mice were killed,and the differences in the expressions of H19,microRNA let-7a(let-7a),phospho-Janus kinase 2(p-JAK2)and phosphorylated signal transducer and activator of transcription-3(p-STAT3)in the colon tissues of the two groups were compared.Results The expression of H19 in the colon of mice in the observation group was higher than that in the control group(P<0.05),and the expression of let-7a in the colon tissue of mice in the observation group was lower than that in the control group(P<0.05).The expression levels of p-JAK2 and p-STAT3 proteins in the colon of mice in the observation group were higher than those in the control group(P<0.05).Conclusion The high expression of H19 in mouse colon cancer tissue is accompanied by the increase of STAT3 expression,which promotes the pathogenesis of CAC.
Ulcerative colitisColorectal cancerLong-chain noncoding RNA H19Signal transducer and activator of transcription-3Inflammation
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