16S-rRNA sequencing technology to analyze the intestinal bacterial genome of premature infants to guide the timing of NEC surgery
Objective To investigate the 16S-rRNA sequencing technology to analyze the intestinal bacterial genome of premature infants to guide the timing of necrotizing enterocolitis of newborn(NEC)surgery.Methods Cases of pediatric patients with NEC admitted to Baise People's Hospital from January 2021 to June 2022 were prospectively selected and divided into the observation group(n=30)and the control group(n=30).The observation group was required to be treated with surgery,while the control group,including 15 cases in phase Ⅰ and 15 case in phase Ⅱa,was conservatively treated in the Department of Internal Medicine during the same period.HiSeq sequencing platform was used,high-throughput second-generation sequencing was carried out with the help of double-end sequencing mode.The diversity index,abundance of dominant species and ratio of different dominant bacteria were compared between the two groups.The receiver operating characteristic(ROC)curves were drawn and the guiding value of 16S-rRNA sequencing technology was analyzed.Results A total of 84 bacteria were obtained from 60 samples of 60 patients,and Parabacteroides was the highest in both groups of species,followed by Ruminococcus,Blautia,Aeromonas and Fusobacterium.There was statistically significant difference in the abundance of the above phylum between the two groups of gut microbiota(P<0.05).A total of 7347 481 valid sequences were obtained from fecal specimens,with an average of 130 857 sequences per capita,and the average sequencing coverage was(92.15±5.61)%.The Shannon-wiener and Simpson diversity index in pediatric patients with NEC treated with surgery in the observation group was lower than that in the control group treated with conservative internal medicine,with statistically significant difference(P<0.05).The ROC curve results showed that the guidance AUC of 16S-rRNA sequencing technology in the selection of surgical timing for pediatric patients with NEC was 0.846,the guidance sensitivity was 87.51%,and the specificity was 83.16%.Conclusion Pediatric patients with NEC are often accompanied by changes in the genome of intestinal bacteria,and the changes in the flora structure are related to the severity of their illness.16S-rRNA sequencing technology can guide the timing of surgical treatment for pediatric patients with NEC.
Necrotizing enterocolitis of newborn16S-rRNA sequencing technologyIntestinal bacterial geneTiming of operation
万方数据
维普
