Experimental research on inhibiting malignant biological behavior of glioblastoma by down-regulating epithelial membrane protein-1
Objective To investigate the impacts of down-regulating epithelial membrane protein-1(EMP1)on proliferation,invasion,migration and apoptosis of U251 and U87 cell lines,and tumor volume and immunohistochemical indices of U87 cell line tumor-bearing mice.Methods The expression of EMP1 in glioblastoma cell lines U87 and U251 was down-regulated by siRNA interference technique.RT-qPCR and Western blot were used to detect the expression of EMP1 mRNA and protein.CCK8 and Transwell experiments were used to detect the proliferation,migration and invasion ability of tumor cells after EMP1 was down-regulated.Flow cytometry was used to detect the apoptosis expression level of tumor cells after EMP1 down-regulation;A nude mouse model of U87 cell line was established,and the expression of Ki-67 and MMP2 was detected by immunohistochemistry.Results RT-qPCR and Western blot results showed that siRNA-EMP1 reduced the expression of EMP1(P＜0.05),and CCK8 and Transwell experiments showed that the proliferation,migration and invasion of tumor cells were significantly inhibited after down-regulating EMP1(P＜0.05).The results of flow cytometry showed that the apoptosis ability of tumor cells was significantly enhanced after down-regulation of EMP1(P＜0.05).Downregulation of EMP1 in the U87 cell line nude mouse tumor-bearing model resulted in delayed tumor cell growth.The expression of Ki-67 and MMP2 was reduced.Conclusion Down-regulation of EMP1 expression can inhibit the proliferation,migration and invasion of glioblastoma,promote apoptosis,delay the growth and invasion of tumor-bearing mice,and thus inhibit the malignant biological behavior of glioblastoma.
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维普
