Objective To conduct in vitro cytotoxicity tests of hydroxypropyl methylcellulose(HPMC)using MTT colorimetry to evaluate the biological safety of HPMC as a pharmaceutical excipient.Methods L929 cells were divided into a blank control group(with only cell culture medium added)and an HPMC group(with two specifications of K4M and K100M,with concentrations of 10.0,5.0,1.0,0.5,and 0.1 mg/ml,respectively).Cultured in an incubator for 24 and 48 h,the absorbance at 490 nm was measured by MTT method,and the relative growth rate(RGR)of cells in cytotoxicity test was calculated.Results The average RGR of HPMCs with different specifications(K4M,K100M)and concentrations(10.0,5.0,1.0,0.5,0.1 mg/ml)was＞85％,indicating stepless cytotoxicity.Conclusion The study results confirm that HPMC has no cytotoxicity on L929 cells,indicating that the cytotoxicity of HPMC meets the requirements of national standards for the cytotoxicity of biomaterials,making it a safe pharmaceutical excipient.
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