Research on the value of different techniques in serological test of hepatitis B virus
Objective To investigate the value of detection techniques of enzyme-linked immunosorbent assay(ELISA)and chemiluminescence enzyme immunoassay(CLIA)in serological test of hepatitis B virus(HBV).Methods A total of 5836 patients with highly suspected HBV infection admitted to the People's Hospital of Liupanshui City from January to December 2022 were selected and included in the research.All patients underwent detections of real-time fluorescence quantitative polymerase chain reaction(qPCR),ELISA and CLIA.The detection results,diagnostic efficiency and positive detection rate of HBV markers between the two detection techniques and qPCR detection were compared.Results After qPCR detection,5836 patients with high suspicion of HBV infection were diagnosed as positive in 3,033 cases and negative in 2803 cases.After CLIA test,2903 cases were diagnosed as positive and 2933 cases as negative,of which only 2790 cases were diagnosed as patients with HBV infection.After ELISA test,2921 cases were diagnosed as positive and 2915 cases as negative,of which only 2731 cases were diagnosed as patients with HBV infection.Compared with ELISA,CLIA had higher sensitivity,specificity,accuracy,positive predictive value,negative predictive value and positive detection rate of hepatitis B surface antigen(HBsAg)and hepatitis B e antigen(HBeAg),with statistically significant differences(P＜0.05).There were no statistically significant differences in the positive detection rates of hepatitis B surface antibody(HBsAb),hepatitis B e antibody(HBeAb)and hepatitis B core antibody(HBcAb)between the two detection methods(P＞0.05).Conclusion Compared with ELISA,CLIA is more effective in serological test of HBV,and can effectively improve the detection rates of HBsAg and HBeAg,with higher diagnostic value.
Hepatitis B virusSerological testEnzyme-linked immunosorbent assayChemiluminescence enzyme immunoassay
万方数据
维普
