Application of metagenomics next-generation sequencing of bronchoalveolar lavage fluid in etiological diagnosis of pulmonary infection
Objective To investigate the application of metagenomics next-generation sequencing of bronchoalveolar lavage fluid in the etiological diagnosis of pulmonary infection.Methods A total of 80 patients with pulmonary infection admitted to Zibo First Hospital from January 2020 to January 2022 were selected as the research object and divided into the observation group(n=40)and the control group(n=40)by drawing lots from the digital table of medical records.All patients underwent the examination of bronchoalveolar lavage fluid,and the observation group was given the metagenomics next-generation sequencing of bronchoalveolar lavage fluid on this basis.The distribution of pathogens in bronchoalveolar lavage fluid,the detection rate of pathogens and the clinical indicators after treatment were compared between the two groups.Results A total of 96 strains of pathogenic bacteria were isolated from the alveolar lavage fluid of 80 patients with pulmonary infection.Thereinto,there were 20 strains of gram-positive bacteria,accounting for 20.83％,mainly Streptococcus pneumoniae and Staphylococcus aureus;there were 72 strains of gram-negative bacteria,accounting for 75.00％,mainly Acinetobacter baumannii,Pseudomonas aeruginosa and Haemophilus influenzae;there were 3 strains of fungi,accounting for 3.12％,mainly Candida albicans.Of the 40 patients in the control group,28 were positive by routine pathogenic bacteria detection,accounting for 70.00％,of the 40 patients in the observation group,37 were positive by metagenomics next-generation sequencing,accounting for 92.50％,which was significantly higher than that in the control group(P＜0.05).The 30-day and 90-day mortalities in the observation group were significantly lower than those in the control group(P＜0.05).Conclusion The metagenomics next-generation sequencing of bronchoalveolar lavage fluid is of great value in the etiological diagnosis of pulmonary infection,which can effectively increase the detection rate and help to give corresponding treatment in clinic.
万方数据
维普
