Component analysis of Atractylodes macrocephala Koidz.and Atractylodes macrocephalacv.Yuzhu from different regions based on liquid chromatography-mass spectrometry pattern recognition
Component analysis of Atractylodes macrocephala Koidz.and Atractylodes macrocephalacv.Yuzhu from different regions based on liquid chromatography-mass spectrometry pattern recognition
Objective To compare and analyze the chemical components of Atractylodes macrocephala Koidz.and Atractylodes macrocephalacv.Yuzhu from different regions,identify the different components,and provide a basis for quality evaluation and authentic research of Atractylodes macrocephala Koidz.and Atractylodes macrocephalacv.Yuzhu.Methods ZORBAX Eclipse XDB-C18 Analytical(4.6 mm × 250 mm,5 μ m)was used as chromatographic column,acetonitrile as mobile phase B,water as mobile phase C,with an elution gradient,column temperature of 35℃ and flow rate of 1 ml/min;UPLC-Triple-TOF 6600+time-of-flight liquid mass spectrometer and positive and negative ion scanning mode were adopted.Combined with chemical pattern recognition cluster analysis,principal component analysis and partial least squares discriminant analysis,the different components of Atractylodes macrocephala Koidz.and Atractylodes macrocephalacv.Yuzhu from different regions were found.Results There were significant differences in the chemical composition of Atractylodes macrocephala Koidz.and Atractylodes macrocephalacv.Yuzhu from different regions,with a total of 5 different components identified,namely AtractylolideⅠ,Atractylochrome D,Isosterolide A,Atractylolide Ⅱ,and Atractylolide Ⅲ.The content of five different components in Atractylodes macrocephala Koidz.was significantly higher than that in other regions.The content of Atractylmacrol D,Isosterolide A,Atractylolide Ⅱ,and Atractylolide Ⅲcomponents in Atractylodes macrocephalacv.Yuzhu was higher than that of Atractylodes macrocephala Koidz.from various regions.Conclusion There are differences in the chemical composition of Atractylodes macrocephala Koidz.and Atractylodes macrocephalacv.Yuzhu from different regions,with significant locality specificity.The effective composition content of Atractylodes macrocephalacv.Yuzhu is better than that of Atractylodes macrocephala Koidz.,while the effective composition content of Zhejiang Atractylodes macrocephala Koidz.is better than that of Atractylodes macrocephala Koidz.from other regions.This study provides a scientific reference for evaluating the quality and geoherbalism of Atractylodes macrocephala Koidz.and Atractylodes macrocephalacv.Yuzhu.
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